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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important position in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been described. Two more splice variants have been reported but specific nucleotide sequence info is not really nevertheless available.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally incorporates the epitope recognized with the antibody.
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This guidebook outlines additional doable optimization of mobile and immunoassay parameters to guarantee the best possible success are...
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This guide outlines more possible optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
Antibodies sure to the blocking peptide no longer bind towards the epitope to the concentrate on protein. By comparing the staining through the blocked antibody vs . the antibody alone, one can see which staining is specific.
Concentrate on Information Cyclin E belongs for the remarkably conserved cyclin family members, whose associates are characterised by a remarkable periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Different cyclins show distinctive expression and degradation designs which add to your temporal coordination of each mitotic celebration. Cyclin E kinds more info a posh with and features like a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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